Insights into the Discrepancy between Single Molecule Experiments *

Qian Zhou,Min Zhang,Yang-Tao Fan,Yu-Kang Wang,Lin Bao,Guang-Ju Zhao,Hu Chen,Yan-Hui Liu

Fig. 3 (Color online) Fork generation at the junction of dsDNA and ssDNA under conditions with kink structure in dsDNA. Loop length of sensor in Figs. 3(a)-3(d) is 37 bp, 57 bp, 81 bp, and 101 bp, respectively, in which the loop is complemented by ssDNA with different lengths to form dsDNA; generation frequency of the fork pertains to contour length of dsDNA in the sensor proportionally. A critical contour length of dsDNA exists in every sensor and their values are approximately 20 bp, 39 bp, 60 bp, and 78 bp, respectively. Fork structure begins to be generated when the contour length of dsDNA in sensor becomes larger than the critical length.